evaluation of chronic hepatitis b infection in patients with seronegative hbsag
نویسندگان
چکیده
background: it is estimated that about 370 million people are chronic carriers of hbv worldwide. apparently 3% of iranian populations are chronic carriers of this virus. we aimed to evaluate the viral dna in biological fluids of chronic hepatitis patients compared to a control group. methods: the current case-control study was designed to evaluate the viral dna in biological fluids of 70 chronic hepatitis patients compared to a control group using elisa, pcr and real time. results: all individuals (100%) in case group were hbsag positive while in control group only 2 individuals (2.8%) were hbsag positive. three individuals , in control group were positive using pcr and real time pcr indicating that about 7% of those in control group were chronic carriers of hbv. the interesting point was the copy of viral dna; (5.49 ×10 4 , 2.162×10 3 and 7.26×10 6 ) for 3 chronic carriers using sera while it was about (5.71×10 3 , 1.45×10 2 and 2.56×10 5 ) using ear cerumen confirming the necessity of investigating for the carriers of hbv in different biological fluid and by different methods. conclusion: it can be concluded chronic carriers of hepatitis b are much more than what is diagnosed by routine diagnostic tests. on the other hand elisa alone can not be relied on as a complete test for screening of chronic carriers in hepatitis b. pcr and real time pcr are more reliable tests for this purpose.
منابع مشابه
Evaluation of Chronic Hepatitis B Infection in Patients with Seronegative HbsAg
BACKGROUND It is estimated that about 370 million people are chronic carriers of HBV worldwide. Apparently 3% of Iranian populations are chronic carriers of this virus. We aimed to evaluate the viral DNA in biological fluids of chronic hepatitis patients compared to a control group. METHODS The current case-control study was designed to evaluate the viral DNA in biological fluids of 70 chroni...
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عنوان ژورنال:
iranian journal of public healthجلد ۴۱، شماره ۲، صفحات ۱۰۰-۱۰۴
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